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Image Search Results
Journal: Assay and Drug Development Technologies
Article Title: Profiling the NIH Small Molecule Repository for Compounds That Generate H 2 O 2 by Redox Cycling in Reducing Environments
doi: 10.1089/adt.2009.0247
Figure Lengend Snippet: Screening the Library of Pharmacologically Active Compounds (LOPAC) set to identify redox cycling compounds (RCCs). (A) The 4 × 384-well plate overlay scatter plot of the % activation of H2O2 production data from the first iteration of the LOPAC screen. An ActivityBase™ HTS template was written to calculate the % activation based on the maximum (n = 32) and minimum (n = 24) plate controls for the HRP-PR H2O2 detection assay. A Spotfire® visualization of the % activation data from 4 × 384-well plates of the HRP-PR H2O2 production data for the first iteration of the LOPAC screen overlaid in a single scatter plot is presented; () maximum (100 μM H2O2 in 1% Dimethyl Sulfoxide (DMSO), n = 32) plate controls, () minimum (1% DMSO, n = 24) plate controls, () 50% (50 μM H2O2 in 1% DMSO, n = 8) plate controls, and () 10 μM compounds in 0.8 mM DTT (1% DMSO, n = 320). (B) Chemical structures, names, and PubChem substance identity numbers (SIDs) of the RCCs identified in the LOPAC set. The chemical structures of 2,3-bis(2-hydroxyethylthio)naphthalene-1,4-dione (NSC 95397, PubChem-SID 400206), and 2,2-dimethyl-3,4-dihydro-2H-benzo[h]chromene-5,6-dione (β-lapachone, PubChem-SID 207115) are presented.
Article Snippet:
Techniques: Activation Assay, Detection Assay